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| HR - XC |
| New innovative SPE phase |
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CHROMABOND® HR-XC, strong mixed-mode polymer-based
cation exchanger for basic analytes
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- mixed-mode SPE phase with distinct cation
exchange and RP properties
excellent enrichment of basic analytes
- modern, spherical support polymer
with optimised pore structure and large surface
good reproducibility, reliable and cost-efficient analyses
- more aggressive washing procedures for matrix
removal possible
cleaner samples and protection of your HPLC and GC instrumentation
- quantification of analytes from heavily contaminated
samples
lower limits of detection also for critical matrices
the perfect fit for basic analytes in combination
with CHROMABOND® HR-X |
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- strong benzenesulphonic acid cation exchanger
exchange capacity 1.0 meq/g
- base material polystyrene-divinylbenzene copolymer
pH stability 1-14
RP capacity 300 mg/g (caffeine in water)
- high-purity material, highest reproducibility
and lowest blank values due to an optimised production process
- spherical particles, particle size 85 µm;
pore size 65-75 Å
very large specific surface 800 m2/g; pore volume 1.4
cm3/g
- outstanding recovery rates especially for the
enrichment of basic analytes
- recommended application:
basic active ingredients from strongly matrix-contaminated samples
like e.g. urine, plasma, serum
fungicides from food
basic analytes like e.g. amines
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Fractionation of acidic, neutral
and basic analytes from serum
MN Appl. No. 304780 |
 |
Column type: CHROMABOND® HR-XC
3 ml/200 mg, Cat. No. 730952
Sample: 1 ml spiked matrix, acidified with 200 µl 2%
H3PO4 Column conditioning: 5 ml MeOH,
5 ml water
Sample application: 1.2 ml spiked serum
Washing: 2 ml 0.1 N HCl in water
Elution: a) 2.5 ml MeOH
(fraction A: neutral and acidic analytes)
b) 5 ml MeOH / 10% NH3
(fraction B: basic analytes)
Peaks:
1. Doxepin
2. Imipramine
3. Amitriptyline
4. Trimipramine
|
| Recovery rate [%] |
| Fraction A: acidic and neutral analytes |
Fraction B: basic analytes |
| Compound |
HR-XC |
Compound |
HR-XC |
Oasis©MCX |
StrataX-C |
| Suprofen |
108 |
Doxepin |
101 |
68 |
82 |
| Naproxen |
85 |
Imipramine |
95 |
71 |
85 |
| Tolmetin |
73 |
Amitriptyline |
94 |
72 |
78 |
| Phenobarbital |
108 |
Trimipramine |
92 |
70 |
81 |
| Indomethacin |
33 |
further analysis: HPLC on NUCLEODUR®
C18
or C8 Gravity, see MN Appl. No. 122230 + 118520 |
| Hexobarbital |
80 |
|
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| similar phases: Oasis® MCX, Strata
X-C, StyreScreen® DBX (trademarks of Waters, Phenomenex®,
UCT) |
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| Download HR-XC brochure here..
(pdf 422kb) |
| |
| CHROMABOND® HR-XC |
Column
Volume ml |
Adsorption
(mg) |
Pack of
(Columns) |
Cat No
(G=Glass) |
CHROMABOND®
|
3 |
200 |
30 |
730952 |
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3 |
500 |
30 |
730953 |
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6 |
500 |
30 |
730955 |
| CHROMAFIX® |
|
155 |
50 |
731755 |
| |
|
240 |
50 |
731756 |
| |
|
500 |
50 |
731757 |
CHROMABOND® Adsorbent
|
|
100 g |
|
730664 |
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| CHROMABOND® |
CHROMAFIX® |
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