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HR - XC
New innovative SPE phase

CHROMABOND® HR-XC, strong mixed-mode polymer-based cation exchanger for basic analytes

  • mixed-mode SPE phase with distinct cation exchange and RP properties
    excellent enrichment of basic analytes

  • modern, spherical support polymer
    with optimised pore structure and large surface

    good reproducibility, reliable and cost-efficient analyses

  • more aggressive washing procedures for matrix removal possible
    cleaner samples and protection of your HPLC and GC instrumentation

  • quantification of analytes from heavily contaminated samples
    lower limits of detection also for critical matrices
… the perfect fit for basic analytes in combination with CHROMABOND® HR-X

 

  • strong benzenesulphonic acid cation exchanger exchange capacity 1.0 meq/g

  • base material polystyrene-divinylbenzene copolymer pH stability 1-14
    RP capacity 300 mg/g (caffeine in water)

  • high-purity material, highest reproducibility and lowest blank values due to an optimised production process

  • spherical particles, particle size 85 µm; pore size 65-75 Å
    very large specific surface 800 m2/g; pore volume 1.4 cm3/g

  • outstanding recovery rates especially for the enrichment of basic analytes

  • recommended application:
    basic active ingredients from strongly matrix-contaminated samples like e.g. urine, plasma, serum
    fungicides from food
    basic analytes like e.g. amines
Fractionation of acidic, neutral and basic analytes from serum
MN Appl. No. 304780


Column type: CHROMABOND® HR-XC
3 ml/200 mg, Cat. No. 730952

Sample: 1 ml spiked matrix, acidified with 200 µl 2% H3PO4 Column conditioning: 5 ml MeOH, 5 ml water

Sample application: 1.2 ml spiked serum

Washing: 2 ml 0.1 N HCl in water

Elution: a) 2.5 ml MeOH
(fraction A: neutral and acidic analytes)
b) 5 ml MeOH / 10% NH3
(fraction B: basic analytes)

Peaks:
1. Doxepin
2. Imipramine
3. Amitriptyline
4. Trimipramine

Recovery rate [%]
Fraction A: acidic and neutral analytes Fraction B: basic analytes
Compound HR-XC Compound HR-XC Oasis©MCX Strata™X-C
Suprofen 108 Doxepin 101 68 82
Naproxen 85 Imipramine 95 71 85
Tolmetin 73 Amitriptyline 94 72 78
Phenobarbital 108 Trimipramine 92 70 81
Indomethacin 33 further analysis: HPLC on NUCLEODUR® C18
or C8 Gravity, see MN Appl. No. 122230 + 118520
Hexobarbital 80
similar phases: Oasis® MCX, Strata™ X-C, StyreScreen® DBX (trademarks of Waters, Phenomenex®, UCT)
 
Download HR-XC brochure here.. (pdf 422kb)
 
CHROMABOND® HR-XC Column
Volume ml
Adsorption
(mg)
Pack of
(Columns)
Cat No
(G=Glass)
CHROMABOND®
3 200 30 730952
  3 500 30 730953
  6 500 30 730955
CHROMAFIX®   155 50 731755
    240 50 731756
    500 50 731757
CHROMABOND® Adsorbent
  100 g   730664
 
   
CHROMABOND® CHROMAFIX®    
   
   
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